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href="../2888/topic-8-metabolism-hl-paper-1-questions.html">Topic 8 Metabolism HL Paper 1 questions</a></label></li><li class=""><label style="padding-left: 0px"><i class="fa fa-fw"></i><a href="../2889/topic-9-plant-biology-hl-paper-1-questions.html">Topic 9 Plant biology HL Paper 1 questions</a></label></li><li class=""><label style="padding-left: 0px"><i class="fa fa-fw"></i><a href="../2890/topic-10-genetics-hl-paper-1-questions.html">Topic 10 Genetics HL Paper 1 questions</a></label></li><li class=""><label style="padding-left: 0px"><i class="fa fa-fw"></i><a href="../2891/topic-11-animal-physiology-hl-paper-1-questions.html">Topic 11 Animal physiology HL Paper 1 questions</a></label></li></ul></div> <div class="hidden-xs hidden-sm"> <button class="btn btn-default btn-block text-xs-center" data-toggle="modal" data-target="#modal-feedback" style="margin-bottom: 10px"><i class="fa fa-send"></i>&nbsp;&nbsp;Feedback</button> </div> </div> <div class="col-md-9" id="main-column"> <h1 class="page_title"> Topic 4 Ecology Paper 1 questions <a href="#" class="mark-page-favorite pull-right" data-pid="2883" title="Mark as favorite" onclick="return false;"><i class="fa fa-star-o"></i></a> </h1> <ol class="breadcrumb"> <li><a href="../../../biology.html"><i class="fa fa-home"></i></a><i class="fa fa-fw fa-chevron-right divider"></i></li><li><a href="../2876/paper-1-style-exam-questions.html">Paper 1 style Exam questions</a><i class="fa fa-fw fa-chevron-right divider"></i></li><li><span class="gray">Topic 4 Ecology Paper 1 questions</span></li> <span class="pull-right" style="color: #555" title="Suggested study time: 30 minutes"><i class="fa fa-clock-o"></i> 30&apos;</span> </ol> <article id="main-article"> <h3><span class="mw-headline" id="Topic_1:_Cell_biology">Topic 4: <a class="internal-link" href="https://e.studyib.net/biology/page/466/04-ecology-">Ecology </a></span> <img alt="" src="../../images/04_ecology/lemur-1.jpg" style="float: right; width: 133px; height: 120px;"></h3> <p><span class="mw-headline"></span> <span class="mw-headline">This page contains<strong> multiple choice questions</strong> in the style of Paper 1 of the Biology exams.<br> They test the breadth of your knowledge of the understandings and skills <strong>about Ecology</strong>.</span><span class="mw-headline"><br> To spend more time reviewing the topic before answering these questions, use the revision resources.</span> <span class="mw-headline"></span></p> <div class="index-list"> <div class="item"> <div class="bg-cover cover-96" onclick="window.location='/biology/page/466/04-ecology-'" style="background-image: url('../../images/04_ecology/greenhouse_globe-1.jpg')" title="04 Ecology ">&nbsp;</div> <div class="content"> <h4 class="head"><a href="../466/04-ecology-.html">Ecology revision resources </a></h4> <p class="body">This is an introduction to the ecology topic. It lists understandings and skills expected for Topic 4 including ecological terminology, species, communities, ecosystems, the nature of energy flow, carbon cycling including carbon reservoirs, carbon...</p> </div> </div> </div> <p><span class="mw-headline"></span><br> <span class="mw-headline">Learn from any mistakes. Every question has an examiner&#39;s explanation that appears when you check your answers.<span class="mw-headline"><span class="mw-headline"><span class="mw-headline"><img alt="" src="../../images/00_random-images/check-button.png" style="width: 80px; height: 35px;"></span></span></span></span></p> <div class="tib-quiz dyn-quiz" data-pid="2883" data-mtime="1675505938.6463"><div class="label label-default q-number">1</div><div class="exercise shadow-bottom"><div class="q-question"><p>Which classification grouping includes all organisms which have the potential to interbreed and produce fertile offspring?</p></div><div class="q-answer"><p><label class="radio"><input type="radio"> Population.</label></p><p><label class="radio"><input type="radio"> Kingdom</label></p><p><label class="radio"><input class="c" type="radio"> Species.</label></p><p><label class="radio"><input type="radio"> Family</label></p></div><div class="q-explanation"><p>Species are &quot;groups of organisms that can potentially interbreed to produce fertile offspring&quot;.</p></div><div class="actions"><span class="score" data-score="0"></span><button class="btn btn-default btn-sm btn-xs-block text-xs-center check"><i class="fa fa-check-square-o"></i> Check</button></div></div><div class="label label-default q-number">2</div><div class="exercise shadow-bottom"><div class="q-question"><p>In an experiment in a small garden, quadrat frames were used to sample the number of clover plants growing in the grass lawn.</p><p><img alt="" src="../../images/2025-richard/c4.2-clover.jpg" style="width: 213px; height: 180px;"></p><p>Why is it important to use random sampling in the placement of the quadrats?</p></div><div class="q-answer"><p><label class="radio"><input type="radio"> Random sampling avoids a normal distribution.</label></p><p><label class="radio"><input type="radio"> Random sampling ensures a large sample.</label></p><p><label class="radio"><input type="radio"> Random sampling is systematic.</label></p><p><label class="radio"><input class="c" type="radio"> Random sampling avoids bias and selection.</label></p></div><div class="q-explanation"><p>Random quadrat sampling is a standard method used to avoid bias and selection.</p></div><div class="actions"><span class="score" data-score="0"></span><button class="btn btn-default btn-sm btn-xs-block text-xs-center check"><i class="fa fa-check-square-o"></i> Check</button></div></div><div class="label label-default q-number">3</div><div class="exercise shadow-bottom"><div class="q-question"><p>In most food webs the transfer of energy from one trophic level to the next is usually about 10%.</p><p>What effect is this likely to have on food chains?</p></div><div class="q-answer"><p><label class="radio"><input type="radio"> Food chains will be shorter than three trophic levels.</label></p><p><label class="radio"><input class="c" type="radio"> Food chains will be limited to no more than five trophic levels.</label></p><p><label class="radio"><input type="radio"> Food chains will become longer and longer.</label></p><p><label class="radio"><input type="radio"> Food chains will become shorter and shorter.</label></p></div><div class="q-explanation"><p>The loss of energy and biomass from trophic level to trophic level is considered to limit the length of food chains. Four trophic levels is common, and five uncommon. It is very rare to have a food web made of chains longer than five trophic levels.</p></div><div class="actions"><span class="score" data-score="0"></span><button class="btn btn-default btn-sm btn-xs-block text-xs-center check"><i class="fa fa-check-square-o"></i> Check</button></div></div><div class="label label-default q-number">4</div><div class="exercise shadow-bottom"><div class="q-question"><p>In ecology a pyramid of energy is often used to show the flow of energy in an ecosystem.</p><p>What does the unit kJ/m<sup>2</sup>/yr represent in such a diagram?</p></div><div class="q-answer"><p><label class="radio"><input type="radio"> The amount of energy in an organism of each trophic level, adjusted to represent 1 m<sup>2</sup> of size per year.</label></p><p><label class="radio"><input type="radio"> The rate of flow of energy standardised for 1m<span tabindex="-1"><sup data-widget="footnotemarker">2</sup><span style="background:rgba(220,220,220,0.5);background-image:url(https://www.e.studyib.net/ckeditor/plugins/widget/images/handle.png)"><img draggable="true" height="15" src="data:image/gif;base64,R0lGODlhAQABAPABAP///wAAACH5BAEKAAAALAAAAAABAAEAAAICRAEAOw==" title="Click and drag to move" width="15"></span></span></label> of ecosystem in a year.</p><p><label class="radio"><input class="c" type="radio"> The amount of energy in organisms of each trophic level harvested over a year in 1 m<sup>2</sup> of ecosystem.</label></p><p><label class="radio"><input type="radio"> The energy trapped from sunlight in 1m<sup>2</sup> of the ecosystem in a year.<span tabindex="-1"><span ckeditor="" handle.png="" https:="" images="" plugins="" style="background: rgba(220, 220, 220, 0.5) url(" widget="" www.e.studyib.net=""><img draggable="true" height="15" src="data:image/gif;base64,R0lGODlhAQABAPABAP///wAAACH5BAEKAAAALAAAAAABAAEAAAICRAEAOw==" title="Click and drag to move" width="15"></span></span></label></p></div><div class="q-explanation"><p>Samples would be taken several times over the year to find the all the organisms in a 1m<span tabindex="-1"><sup data-widget="footnotemarker">2</sup><span style="background:rgba(220,220,220,0.5);background-image:url(https://www.e.studyib.net/ckeditor/plugins/widget/images/handle.png)"><img draggable="true" height="15" src="data:image/gif;base64,R0lGODlhAQABAPABAP///wAAACH5BAEKAAAALAAAAAABAAEAAAICRAEAOw==" title="Click and drag to move" width="15"></span></span> quadrat.<br>The energy contained in the biomass of these organisms will be measured (or calculated).<br>Kilojoules are the units of energy.</p></div><div class="actions"><span class="score" data-score="0"></span><button class="btn btn-default btn-sm btn-xs-block text-xs-center check"><i class="fa fa-check-square-o"></i> Check</button></div></div><div class="label label-default q-number">5</div><div class="exercise shadow-bottom"><div class="q-question"><p>What is the usual source of energy in ecosystems?</p></div><div class="q-answer"><p><label class="radio"><input type="radio"> The energy comes from the inorganic nutrients.</label></p><p><label class="radio"><input type="radio"> The energy is recycled with the inorganic nutrients.</label></p><p><label class="radio"><input class="c" type="radio"> The energy of sunlight.</label></p><p><label class="radio"><input type="radio"> The energy is released from chemicals in rocks and soil.</label></p></div><div class="q-explanation"><p>The sun provides energy to ecosystems, continuously, and it is trapped in organic molecules by photosynthesis by autotrphs. Inorganic nutrients are recycled by decomposers but when they are reabsorbed into plants they do not provide energy.</p><p>Some chemoautotrophs can use inorganic energy from mineral elements but the great majority of autotrophs use light.</p></div><div class="actions"><span class="score" data-score="0"></span><button class="btn btn-default btn-sm btn-xs-block text-xs-center check"><i class="fa fa-check-square-o"></i> Check</button></div></div><div class="label label-default q-number">6</div><div class="exercise shadow-bottom"><div class="q-question"><p>This diagram shows a simple model of energy flow.</p><p><img alt="" src="../../images/04_ecology/foodweb_fish_redblobs.png" style="width: 400px; height: 222px;"></p><p>Which of the organisms could be called &quot;secondary consumers&quot;?</p></div><div class="q-answer"><p><label class="radio"><input type="radio"> Large phytoplankton and large zooplankton.</label></p><p><label class="radio"><input type="radio"> Large zooplankton and small zooplankton.</label></p><p><label class="radio"><input class="c" type="radio"> Large zooplankton and fish.</label></p><p><label class="radio"><input type="radio"> Small phytoplankton and small zooplankton.</label></p></div><div class="q-explanation"><p>Producers are photosynthetic, primary consumers eat producers, and secondary consumers eat primary consumers. The situation is complicated this this model, because the large zooplankton could be described as a primary consumer or a secondary consumer, because it eats large phytoplankton which is a producer and small zooplankton.</p></div><div class="actions"><span class="score" data-score="0"></span><button class="btn btn-default btn-sm btn-xs-block text-xs-center check"><i class="fa fa-check-square-o"></i> Check</button></div></div><div class="label label-default q-number">7</div><div class="exercise shadow-bottom"><div class="q-question"><p>In what forms is Carbon present in aquatic ecosystems, and available for autotrophs to use in photosynthesis?</p></div><div class="q-answer"><p><label class="radio"><input type="radio"> As dissolved CO<sub>2 </sub>and carbonic acid.</label></p><p><label class="radio"><input type="radio"> As dissolved CO<sub>2</sub> and calcium carbonate</label></p><p><label class="radio"><input class="c" type="radio"> As dissolved CO<sub>2</sub> and hydrogen carbonate ions. </label></p><p><label class="radio"><input type="radio"> As hydrogen carbonate ions and as calcium carbonate</label></p></div><div class="q-explanation"><p>In the syllabus carbon is said to be present as dissolved carbon dioxide and hydrogen carbonate ions.</p></div><div class="actions"><span class="score" data-score="0"></span><button class="btn btn-default btn-sm btn-xs-block text-xs-center check"><i class="fa fa-check-square-o"></i> Check</button></div></div><div class="label label-default q-number">8</div><div class="exercise shadow-bottom"><div class="q-question"><p>Methane (CH<sub>4</sub>) is produced in some situations in ecosystems.<br>When does this happen?</p></div><div class="q-answer"><p><label class="radio"><input type="radio"> In aerobic conditions in soil on agricultural land.</label></p><p><label class="radio"><input class="c" type="radio"> In anaerobic conditions in waterlogged soil.</label></p><p><label class="radio"><input type="radio"> In aerobic conditions in bacteria in water.</label></p><p><label class="radio"><input type="radio"> In anaerobic conditions when photosynthesis occurs.</label></p></div><div class="q-explanation"><p>Methane is produced from decaying organic matter in anaerobic conditions by bacteria.<br>This is common in the formation of peat, in waterlogged soils.</p></div><div class="actions"><span class="score" data-score="0"></span><button class="btn btn-default btn-sm btn-xs-block text-xs-center check"><i class="fa fa-check-square-o"></i> Check</button></div></div><div class="label label-default q-number">9</div><div class="exercise shadow-bottom"><div class="q-question"><p><span style="font-size:13px;color:#000000;font-weight:normal;text-decoration:none;font-family:'Arial';font-style:normal;text-decoration-skip-ink:none;">Why would a &Chi;<sup>2</sup> (chi squared) test be applied to the data for a count of a plant species in the trampled area of a field in comparison to a grazed area?</span></p></div><div class="q-answer"><p><label class="radio"><input type="radio"> To support the hypothesis.</label></p><p><label class="radio"><input type="radio"> To determine whether there is a difference between the populations in each area.</label></p><p><label class="radio"><input type="radio"> To determine whether the population values are accurate.</label></p><p><label class="radio"><input class="c" type="radio"> To determine whether there is a significant difference between the populations in each area.</label></p></div><div class="q-explanation"><p>Statistical tests are applied to ascertain if the difference between two situations are a significant difference. The test applied may or may not support the hypothesis.</p></div><div class="actions"><span class="score" data-score="0"></span><button class="btn btn-default btn-sm btn-xs-block text-xs-center check"><i class="fa fa-check-square-o"></i> Check</button></div></div><div class="label label-default q-number">10</div><div class="exercise shadow-bottom"><div class="q-question"><p>Which of the following forms of energy can be the primary energy source for an ecosystem I heat II light III chemical IV electrical?</p></div><div class="q-answer"><p><label class="radio"><input type="radio"> II only</label></p><p><label class="radio"><input type="radio"> I,II,III only</label></p><p><label class="radio"><input class="c" type="radio"> II,III only</label></p><p><label class="radio"><input type="radio"> All of the above</label></p></div><div class="q-explanation"><p>Most ecosystems depend upon light energy as a primary source but some can use chemical energy (particularly Archaeans living in extreme environments such as thermal vents, deep sea)</p></div><div class="actions"><span class="score" data-score="0"></span><button class="btn btn-default btn-sm btn-xs-block text-xs-center check"><i class="fa fa-check-square-o"></i> Check</button></div></div><div class="label label-default q-number">11</div><div class="exercise shadow-bottom"><div class="q-question"><p>What is a definition of the biomass of a population?</p></div><div class="q-answer"><p><label class="radio"><input type="radio"> The total mass of of living tissue in an organism at a certain time</label></p><p><label class="radio"><input type="radio"> The number of organisms in a population at a certain time.</label></p><p><label class="radio"><input type="radio"> The total mass of of living tissue in a community at a certain time.</label></p><p><label class="radio"><input class="c" type="radio"> The total mass of of living tissue in a population at a certain time.</label></p></div><div class="q-explanation"><p>Biomass refers to the mass of living tissue in a population.</p></div><div class="actions"><span class="score" data-score="0"></span><button class="btn btn-default btn-sm btn-xs-block text-xs-center check"><i class="fa fa-check-square-o"></i> Check</button></div></div><div class="label label-default q-number">12</div><div class="exercise shadow-bottom"><div class="q-question"><p>How could the biomass of a population at the second <span class="scayt-misspell-word" data-scayt-word="trophic" data-wsc-id="kul6s6yrtz7qkn169" data-wsc-lang="en_GB">trophic</span> level be measured?</p></div><div class="q-answer"><p><label class="radio"><input type="radio"> Capture all the organisms and weigh them.</label></p><p><label class="radio"><input type="radio"> Capture and release a representative number of organisms and estimate the population size.</label></p><p><label class="radio"><input class="c" type="radio"> Estimate the population size and either research an average mass or measure the mass of the organisms.</label></p><p><label class="radio"><input type="radio"> Estimate the population size.</label></p></div><div class="q-explanation"><p>An estimate is taken from a representative sample. From this an estimate of the size of the population is made and then a measure of the mass or the sample captured.</p></div><div class="actions"><span class="score" data-score="0"></span><button class="btn btn-default btn-sm btn-xs-block text-xs-center check"><i class="fa fa-check-square-o"></i> Check</button></div></div><div class="label label-default q-number">13</div><div class="exercise shadow-bottom"><div class="q-question"><p>Recycling of inorganic substances can be on a global or local scale. Which of the following are recycled on a global scale? I Energy II Carbon III Oxygen IV Water.</p></div><div class="q-answer"><p><label class="radio"><input type="radio"> II only</label></p><p><label class="radio"><input class="c" type="radio"> II,III, IV only</label></p><p><label class="radio"><input type="radio"> IV only</label></p><p><label class="radio"><input type="radio"> II, IV only</label></p></div><div class="q-explanation"><p>Carbon dioxide, oxygen and water are recycled, energy cannot be recycled. As the cycles have a gaseous component, the cycle is global rather than local.</p></div><div class="actions"><span class="score" data-score="0"></span><button class="btn btn-default btn-sm btn-xs-block text-xs-center check"><i class="fa fa-check-square-o"></i> Check</button></div></div><div class="label label-default q-number">14</div><div class="exercise shadow-bottom"><div class="q-question"><p>In terms of radiation, reflection and absorbtion, which is the best explanantion of the greenhouse effect?</p></div><div class="q-answer"><p><label class="radio"><input type="radio"> Long-wave radiation from the sun strikes the earth and is reflected as short-wave radiation which is absorbed by the greenhouse layer</label></p><p><label class="radio"><input type="radio"> Short-waveradiation from the sun strikes the earth and is reflected as ultra-violet radiation which is absorbed by the greenhouse layer</label></p><p><label class="radio"><input type="radio"> Short-waveradiation from the sun strikes the earth and is reflected as long-wave radiation which passes through the greenhouse layer</label></p><p><label class="radio"><input class="c" type="radio"> Short wave-radiation from the sun strikes the earth and is reflected as long-wave radiation which is absorbed by the greenhouse layer</label></p></div><div class="q-explanation"><p>Most of the short wave radiation from the sun that strikes the earth is converted to heat. Some radiation is reflected as long-wave radiation, much of which is trapped in the greenhouse layer causing a heating effect</p></div><div class="actions"><span class="score" data-score="0"></span><button class="btn btn-default btn-sm btn-xs-block text-xs-center check"><i class="fa fa-check-square-o"></i> Check</button></div></div><div class="label label-default q-number">15</div><div class="exercise shadow-bottom"><div class="q-question"><p>Why is it difficult to prove a direct link between climate change and human activities increasing carbon dioxide concentrations in the atmosphere?</p></div><div class="q-answer"><p><label class="radio"><input type="radio"> There is a lack of data</label></p><p><label class="radio"><input type="radio"> They are not related</label></p><p><label class="radio"><input type="radio"> The evidence is not sufficient</label></p><p><label class="radio"><input class="c" type="radio"> There are many factors that can affect the climate</label></p></div><div class="q-explanation"><p>It is difficult for scientists to demonstrate a direct link as there are many confounding variables which can affect the climate</p></div><div class="actions"><span class="score" data-score="0"></span><button class="btn btn-default btn-sm btn-xs-block text-xs-center check"><i class="fa fa-check-square-o"></i> Check</button></div></div><div class="label label-default q-number">16</div><div class="exercise shadow-bottom"><div class="q-question"><p>The graph illustrates global mean annual temperatures between 1880 and 2018 shown in comparison to the overall mean annual temperature during this period.</p><p><img alt="" src="../../images/2025-richard/4.4-climate-change.jpg" style="width: 268px; height: 180px;"></p><p>What is the difference between the lowest and the highest mean annual temperature shown on the graph?</p></div><div class="q-answer"><p><label class="radio"><input type="radio"> 1.4<sup>0</sup>C</label></p><p><label class="radio"><input type="radio"> 0.6<sup>0</sup>C </label></p><p><label class="radio"><input class="c" type="radio"> 2.2<sup>0</sup>C</label></p><p><label class="radio"><input type="radio"> 2.5<sup>0</sup>C</label></p></div><div class="q-explanation"><p>The lowest is -0.8<sup>0</sup>C and the highest is 1.4<sup>0</sup>C totalling 2.2<sup>0</sup>C</p></div><div class="actions"><span class="score" data-score="0"></span><button class="btn btn-default btn-sm btn-xs-block text-xs-center check"><i class="fa fa-check-square-o"></i> Check</button></div></div><div class="label label-default q-number">17</div><div class="exercise shadow-bottom"><div class="q-question"><p>Which is the best definition of the term, &quot;heterotrophic&quot;</p></div><div class="q-answer"><p><label class="radio"><input type="radio"> Organisms which use photosynthesis to make energy rich organic molecules.</label></p><p><label class="radio"><input type="radio"> Organisms which eat dead or decaying organic material by external digestion.</label></p><p><label class="radio"><input type="radio"> Organisms which are eaten by other living organisms.</label></p><p><label class="radio"><input class="c" type="radio"> Organisms which ingets and internally digest other living organisms.</label></p></div><div class="q-explanation"><p>Heterotrophs need to obtain organic material from other organisms. They cannot produce organic material from inorganic molecules. They ingest and internally digest other living organisms.</p></div><div class="actions"><span class="score" data-score="0"></span><button class="btn btn-default btn-sm btn-xs-block text-xs-center check"><i class="fa fa-check-square-o"></i> Check</button></div></div><div class="label label-default q-number">18</div><div class="exercise shadow-bottom"><div class="q-question"><p>In an experiment in a small garden, quadrat frames were used to sample the number of clover plants growing in the grass lawn.</p><p><img alt="" src="../../images/2025-richard/c4.2-clover.jpg" style="width: 213px; height: 180px;"></p><p>Why are quadrats used to estimate population size?</p></div><div class="q-answer"><p><label class="radio"><input type="radio"> Random sampling allows an eastimate of a normal distribution.</label></p><p><label class="radio"><input type="radio"> Random sampling ensures a small sample.</label></p><p><label class="radio"><input type="radio"> Random sampling is completely accurate.</label></p><p><label class="radio"><input class="c" type="radio"> Taking a sample is a realistic means of estimating population in a reasonable time frame.</label></p></div><div class="q-explanation"><p>Population size done by counting the whole population is generally too time consuming.</p></div><div class="actions"><span class="score" data-score="0"></span><button class="btn btn-default btn-sm btn-xs-block text-xs-center check"><i class="fa fa-check-square-o"></i> Check</button></div></div><div class="label label-default q-number">19</div><div class="exercise shadow-bottom"><div class="q-question"><p>When members of a species are separated by a physical barrier this can lead to populations which are prevented from reproducing, because individuals from each population never meet.</p><p>What is the name given to this situation?</p></div><div class="q-answer"><p><label class="radio"><input type="radio"> Artificial segregation. </label></p><p><label class="radio"><input type="radio"> Artificial isolation</label></p><p><label class="radio"><input type="radio"> Reproductive segregation.</label></p><p><label class="radio"><input class="c" type="radio"> Reproductive isolation</label></p></div><div class="q-explanation"><p>Populations of a single species may be reproductively isolated and form separate populations.</p></div><div class="actions"><span class="score" data-score="0"></span><button class="btn btn-default btn-sm btn-xs-block text-xs-center check"><i class="fa fa-check-square-o"></i> Check</button></div></div><div class="label label-default q-number">20</div><div class="exercise shadow-bottom"><div class="q-question"><p>What trends in the energy available are seen at successive levels in food chains?</p></div><div class="q-answer"><p><label class="radio"><input type="radio"> Energy available increases at successive levels in a food chain.</label></p><p><label class="radio"><input type="radio"> Energy available is roughly constant at successive levels in a food chain.</label></p><p><label class="radio"><input class="c" type="radio"> Energy available decreases at successive levels in a food chain.</label></p><p><label class="radio"><input type="radio"> They are positively correlated.</label></p></div><div class="q-explanation"><p>Energy lost at one level of a food chain is not available for the next level so the energy available in the orgainsms at each successive level will decrease.</p></div><div class="actions"><span class="score" data-score="0"></span><button class="btn btn-default btn-sm btn-xs-block text-xs-center check"><i class="fa fa-check-square-o"></i> Check</button></div></div><div class="totals"><span class="score"></span><button class="btn btn-success btn-block text-center check-total"><i class="fa fa-check-square-o"></i> Check</button></div></div><hr> <p>&nbsp;Refresh this page to try a new set of 20 multiple choice questions. 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